Steffen Möller, Pablo Serrano-Fernández, Michael Watson, Dirk Koczan, Vassilis Kotsikoris, Manfred Kunz, Martin Blüggel, Jens Pahnke, Harald Illges, Stefan Mikkat, Hans-Jürgen Thiesen, Michael O. Glocker, Uwe K. Zettl, Saleh M. Ibrahim:
Predicting soluble variants of membrane proteins.

PSB2005 5 (0028), 2005
© Bioinformation Systems e.V.

The existence of a soluble splice variant for a gene encoding a transmembrane protein suggests that this gene plays a role in intercellular signalling, particularly in immunological processes. Also, the absence of a splice variant of a reported soluble variant suggests exclusive control of the solubilisation by proteolytic cleavage. Soluble splice variants of membrane proteins may also be interesting targets for crystallisation as their structure may be expected to preserve, at least partially, their function as integral membrane proteins, whose structures are most difficult to determine. This paper presents a dataset derived from the literature in an attempt to collect all reported soluble variants of membrane proteins, be they splice variants or shedded. A list of soluble variants is derived in silico from Ensembl. These are checked on their presence in multiple organisms and their number of membranespanning regions is inspected. The findings then are confirmed by a comparison with identified proteins of a recent global proteomics study of human blood plasma. Finally, a tool to determine novel soluble variants by proteomics is provided.



	author = {Steffen M\"oller and Pablo Serrano-Fernández and Michael Watson and Dirk Koczan and Vassilis Kotsikoris and Manfred Kunz and Martin Bl\"uggel and Jens Pahnke and Harald Illges and Stefan Mikkat and Hans-J\"urgen Thiesen and Michael O. Glocker and Uwe K. Zettl and Saleh M. Ibrahim},
	title = {Predicting soluble variants of membrane proteins},
	journal = {PSB2005},
	year = {2005},
	volume = {5},
	number = {0028},
	url = {}